Atherobesity - DFG KFO 152 "Fett und Gefäß"

Subproject 6: The Role of Repin1 in the Pathogenesis of Obesity

Principal Investigators:

 

PD Dr. rer. med. Nora Klöting Prof. Dr. med. Michael Stumvoll
Integrated Research and Treatment Center (IFB) "AdiposityDiseases" Endocrinology and Nephrology Clinic
Universität Leipzig Universität Leipzig
Liebigstraße 21 Liebigstraße 20
04103 Leipzig 04103 Leipzig
Phone: +49 341 97 13401 Phone: +49 341 97 13380
Fax: +49 341 97 13409 Fax: +49 341 97 13389
nora.kloeting@medizin.uni-leipzig.de michael.stumvoll@medizin.uni-leipzig.de

 

Summary of the Subproject:

During the first funding period, in subproject 3 (Heterogenity of Adipose Tissue, Prof. Dr. med. Matthias Blüher), the replication initiating factor 1 (repin1) has been identified as a yet unknown candidate molecule for the development of visceral obesity. In the preliminary work for this subproject, we were able to show the functional role of Repin1 in adipogenesis, regulation of glucose uptake and fat retention in 3T3L1-cells  in vitro. Therefore, we generated a repin1 knock out (Repin1KO) mouse to investigate the role of repin1 in the development of obesity and concomitant diseases in vivo. The aim of this subproject is the comprehensive characterization of the Repin1KO mouse and the conditional, liver-specific Repin1 knock out using Albumin-Cre. This subproject will test the hypothesis that Repin1KO mice are protected against obesity and its concomitant diseases and that the liver is an essential organ for Repin1 function.

Publications:

  1. Kern M, Kovacs P, Fasshauer M, Klöting I, Fitzl G, Blüher M, Stumvoll M, Klöting N. Defizienz des Replikationsinitiator 1 in der Leber führt zur Ausprägung einer Dyslipidämie in vivo. Diabetologie & Stoffwechsel. 2010;5:S20.
  2. Ruschke K, Illes M, Kern M, Klöting I, Fasshauer M, Schön MR, Kosacka J, Fitzl G, Kovacs P, Stumvoll M, Blüher M, Klöting N. Repin1 maybe involved in the regulation of cell size and glucose transport in adipocytes. Biochem Biophys Res Commun. 2010;400:246-51.
  3. Klöting N, Kovacs P, Kern M, Heiker JT, Fasshauer M, Schön MR, Stumvoll M, Beck-Sickinger AG, Blüher M. Central vaspin administration acutely reduces food intake and has sustained blood glucose-lowering effects. Diabetologia. 2011;54:1819-23.
  4. Kern M, Knigge A, Heiker JT, Kosacka J, Stumvoll M, Kovacs P, Blüher M, Klöting N. C57BL/6JRj mice are protected against diet induced obesity (DIO). Biochem Biophys Res Commun. 2012;417:717-20.
  5. Heiker JT, Klöting N. Replication initiator 1 in adipose tissue function and human obesity. Vitam Horm. 2013;91:97-105.
  6. Heiker JT, Kern M, Kosacka J, Flehmig G, Stumvoll M, Shang E, Lohmann T, Dreßler M, Kovacs P, Blüher M, Klöting N. Nicotinamide nucleotide transhydrogenase mRNA expression is related to human obesity. Obesity (Silver Spring). 2013;21:529-34.
  7. Kosacka J, Koch K, Gericke M, Nowicki M, Heiker JT, Klöting I, Stumvoll M, Blüher M, Klöting N. The polygenetically inherited metabolic syndrome of male WOKW rats is associated with enhanced autophagy in adipose tissue. Diabetol Metab Syndr. 2013;5:23.
  8. Heiker JT, Klöting N, Kovacs P, Kuettner EB, Sträter N, Schultz S, Kern M, Stumvoll M, Blüher M, Beck-Sickinger AG. Vaspin inhibits kallikrein 7 by serpin mechanism. Cell Mol Life Sci. 2013;70:2569-83.
Imprint | Contact | © 2014 Author: Prof. Dr. M. Blüher